Pellet protein G magnetic beads by placing the tubes in a magnetic separation rack and wait 1 to 2 min for solution to clear Carefully transfer eluted chromatin supernatant to a new tube To all tubes including the 2 input sample from Step 1 reverse crosslinks by adding 6 181l 5M NaCl and 2 181l Proteinase K 10012 and incubate 2 h at 65176C
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Pellet protein G magnetic beads by placing the tubes in a magnetic separation rack and wait 1 to 2 min for solution to clear Carefully transfer eluted chromatin supernatant to a new tube To all tubes including the 2 input sample from Step 1 reverse crosslinks by adding 6 181l 5M NaCl and 2 181l Proteinase K 10012 and incubate 2 h at 65176C
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